Software

CanProVar

  • Description: CanProVar is designed to store and display single amino acid alterations including both germline and somatic variations in the human proteome, especially those related to the genesis or development of human cancer based on the published literatures. Cancer-related variations and conrresponding annotations can be queried through the web-interface using Protein IDs in the Ensembl, IPI, RefSeq, and Uniport/Swiss-Prot databases or gene names and Entrez gene IDs. Fasta files with variation information are also available for download.
  • URLs:  http://canprovar.zhang-lab.org/ (version 1);  http://canprovar2.zhang-lab.org/ (version 2)
  • Reference:  Jing Li, Dexter T Duncan, Bing Zhang. CanProVar: a human cancer proteome variation database. Hum Mutat,31(3):219-228, 2010

CEA

  • Description: CEA (Complex-Enrichment Analysis) uses a protein interaction network-assisted approach to improve protein identification in shotgun proteomics. A large proportion of possible proteins are eliminated as a result of insufficient experimental evidence in shotgun proteomics data analysis. CEA can be used to rescue the eliminated proteins based on a simple assumption: possible proteins are more likely to be present in the original sample if they exist in a complex enriched with confidently identified proteins. In various data sets tested, CEA increased protein identificaion by 10-30% with an estimated accuracy of 85%.
  • URL: http://bioinfo.vanderbilt.edu/cea
  • Reference: Jing Li, Lisa J Zimmerman, Byung-Hoon Park, David L Tabb, Daniel C Liebler, Bing Zhang. Network-assisted protein identification and data interpretation in shotgun proteomics. Mol Syst Biol, 5:303, 2009

customProDB

GLAD4U

  • Description: The goal of Gene List Automatically Derived For You (GLAD4U) is to implement an algorithm searching the scientific literature (Pubmed) to retrieve the list of publications corresponding to a user’s query. The algorithm will then translate the list of publications into a list of genes referenced in these publications. The last step is to present the user with the list of prioritized genes, from the most to the least referenced genes within the search space.
  • URL: http://glad4u.zhang-lab.org
  • Reference: Jerome Jourquin, Dexter Duncan, Zhiao Shi, Bing Zhang. GLAD4U: deriving and prioritizing gene lists from PubMed literature. BMC Genomics. 13(Suppl 8):S20, 2012.

GPU-FAN

  • Description: Network analysis plays an important role in systems biology. However, network analysis algorithms are usually computationally intensive. Mordern General Purpose computation on Graphics Processing Units (GPGPUs) provides a cost-effective platform for this type of applications. We have initiated a project to enable fast network analysis on GPUs. The first verison of the software package gpu-fan (GPU-based Fast Analysis of Networks) includes methods for computing four shortest path-based centrality metrics on NVIDIA’s CUDA platform. Speedup of 10x ~ 50x was observed for simulated scale-free networks and real world protein interaction and gene co-expression networks.
  • URL: http://gpu-fan.zhang-lab.org
  • Reference: Zhiao Shi, Bing Zhang. Fast network centrality analysis using GPUs. BMC Bioinformatics, 12:149, 2011

GOTM (GOTM has been merged into WebGestalt in 2010)

ICE

LinkedOmics

  • Description: LinkedOmics is publicly available portal that includes multi-omics data from all 32 TCGA Cancer types. It also includes mass spectrometry-based proteomics data generated by the Clinical Proteomics Tumor Analysis Consortium (CPTAC) for TCGA breast, colorectal and ovarian tumors. The web application has three analytical modules: LinkFinder, LinkInterpreter and LinkCompare. LinkFinder allows users to search for attributes that are associated with a query attribute, such as mRNA or protein expression signatures of genomic alterations, candidate biomarkers of clinical attributes, and candidate target genes of transcriptional factors, microRNAs, or protein kinases. Analysis results can be visualized by scatter plots, box plots, or Kaplan-Meier plots. To derive biological insights from the association results, the LinkInterpreter module performs enrichment analysis based on Gene Ontology, biological pathways, network modules, among other functional categories. The LinkCompare module uses visualization functions (interactive venn diagram, scatter plot, and sortable heat map) and meta-analysis to compare and integrate association results generated by the LinkFinder module, which supports multi-omics analysis in a cancer type or pan-cancer analysis.
  • URLs: http://linkedomics.org/
  • Reference: Vasaikar S., Straub P., Wang J., Zhang B. LinkedOmics: analyzing multi-omics data within and across 32 cancer types. Nucleic Acids Research, gkx1090, 2017, https://doi.org/10.1093/nar/gkx109

NetGestalt

NetSAM

  • Description: NetSAM (Network Seriation and Modularization) is an R package that takes an edge-list representation of a network as an input and generates files that can be used as an input for the one-dimensional network visualization tool NetGestalt (http://www.netgestalt.org) or other network analysis. NetSAM uses random walk distance-based hierarchical clustering to identify the hierarchical modules of the network (network modularization) and then uses the optimal leaf ordering (OLO) method to optimize the one-dimensional ordering of the genes in each module by minimizing the sum of the pair-wise random walk distance of adjacent genes in the ordering (network seriation).
  • URL: http://www.bioconductor.org/packages/release/bioc/html/NetSAM.html
  • Reference: Zhiao Shi, Jing Wang, Bing Zhang. NetGestalt: integrating multidimensional omics data over biological networks. Nature Methods, 10:597-598, 2013

NetWalker

  • Description: NetWalker takes a network and a list of nodes from the network as input and calculates steady-state probabilities (final scores) for all nodes in the network based on the random walk technology. Statistical analysis is implemented to evaluate the significance of the final scores. Specifically, for each node, a global p value is calculated to evaluate the overall significance of the node with regard to the input nodes, while a local p value is calculated to ensure that the significance is not simply due to network topology.
  • URL: http://netwalker.zhang-lab.org
  • Reference: Bing Zhang, Zhiao Shi, Dexter T. Duncan, Naresh Prodduturi, Lawrence J Marnett, Daniel C Liebler. Relating protein adduction to gene expression changes: a systems approach. Molecular Biosystems. 2011

PepQuery

  • Description: PepQuery is a peptide-centric search engine that allows quick and easy proteomic validation of genomic alterations without customized database construction. Next generation sequencing-based genomic studies continuously identify new genomic alterations that may lead to novel protein sequences, which are attractive candidates for disease biomarkers and therapeutic targets after proteomic validation. The popular approach for proteomic validation requires customized database construction and a full evaluation of all possible spectrum-peptide pairs, which is time-consuming. We implemented PepQuery as both stand-alone and web-based applications. The stand-alone version supports batch analysis and user-provided MS/MS data. The web version provides access to more than half a billion MS/MS spectra from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) and other cancer proteomic studies, making MS/MS data directly available and useful to scientists outside the proteomics community.
  • URL: http://pepquery.org
  • Reference:  Wen, B., et al. (2019). “PepQuery enables fast, accurate, and convenient proteomic validation of novel genomic alterations.” Genome Res 29(3): 485-493. Manuscript.

WebGestalt